Characterization of Ralstonia solanacearum causing bacterial wilt from major chili growing areas of Pakistan

For proper disease management, accurate diagnosis of the pathogen is essential. Therefore, in present study Ralstonia solanacearum causing bacterial wilt chili was characterized to determine distribution biovars bacterium eight agroecological zones with varying climatic conditions and edaphic factors. Among all 114 isolates R. solanacearum, 77% showed mucoid growth while 23% gave non-mucoid growth. Similarly, were found positive for hypersensitivity response (HR), those negative HR. All grew well at 37 °C, none produced its colony 41 °C. responses biochemical tests used confirmation bacterium. Out isolates, 81% identified as Biovar III remaining 19% recognized IV. constituted 70% provinces Punjab Sindh, respectively, IV formed 19 30%. On other hand, Khyber Pakhtunkhwa Balochistan, only recorded. observed from four country be predominant. recorded located Sindh. yielded a 750-bp band that corresponded solanacearum. It concluded widely prevalent warranting stringent control measures.